Proteome-wide chemical cross-linking combined with mass spectrometry (XL-MS) is an untargeted large-scale approach to capture protein structures and interactions in a physiologically relevant context. By applying XL-MS to synaptosome and microsome fractions purified from hippocampus and cerebellum of mouse brain, we generated a protein interaction resource from the 11,999 cross-links identified. Proteins in close proximity are connected by cross-linking reagents in their subcellular compartments in order to reveal potential protein-binding interfaces and novel partnerships (by inter-protein cross-links) as well as elucidate protein conformations (by intra-protein cross-links). This website works best with the Google Chrome or Firefox browser, Internet Explorer is not supported.
Gonzalez-Lozano, M. A., et al. "Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions." Science Advances 6.8 (2020): eaax5783. DOI: 10.1126/sciadv.aax5783
Reference for use of the data
Gonzalez-Lozano, M. A., et al. "Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions." Science Advances 6.8 (2020): eaax5783. DOI: 10.1126/sciadv.aax5783
Correspondence to A.B. Smit: guus.smit [at] vu.nl
Department of Molecular and Cellular
Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije
Universiteit, Amsterdam, The Netherlands.
https://mcn.cncr.nl
Protein cross-linking web resource
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